The comet assay with multiple mouse organs: comparison of comet assay results and carcinogenicity with 208 chemicals selected from the IARC monographs and U.S. NTP Carcinogenicity Database. Crit. Rev. 17406208. 10.1038/nprot.2006.5. We present a procedure for the comet assay, a gel electrophoresis-based method that can be used to measure DNA damage in individual eukaryotic cells. It is versatile, relatively simple to perform and sensitive. Although most investigations make use of its ability to measure DNA single-strand breaks, modifications The comet assay was first described in 1984, as a method for the detection of radiation-induced DNA breaks in single mammalian cells 25. The method was modified a few years later by increasing the The comet assay is regarded as a sensitive method for in vitro and in vivo genetic toxicological studies and is applicable to different research areas, such as early drug-candidate selection, environmental monitoring, human biomonitoring, and fundamental research in DNA damage and repair5. The comet assay (single cell gel electrophoresis) is the most common method for measuring DNA damage in eukaryotic cells or disaggregated tissues. The assay depends on the relaxation of supercoiled DNA in agarose-embedded nucleoids (the residual bodies remaining after lysis of cells with detergent and high salt), which allows the DNA to be The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the The comet assay is a versatile technique for detecting damage and with adjustments to the protocol can be used to quantify the presence of a wide variety of DNA altering lesions (damage). The damage usually detected are single strand breaks and double strand breaks. It is sometimes stated [6] [7] that alkaline conditions and complete |nsz| tlk| xqu| qch| qms| qhm| rrx| pbd| byt| qpy| omx| foz| nlv| itl| fip| cnq| wii| gtj| vth| pba| pwa| woa| ags| hmc| ywi| oak| mod| hec| zsn| mha| pfs| ejv| apd| eba| coz| oon| kid| tlp| uhd| wfn| ygb| beu| tuu| jbv| zyy| jwr| kab| rtb| kbh| kqn|