FAQ. Gateway™ LR Clonase™ II enzyme mix는 expression clone을 생성하는데 entry clone ( att L site에 flank된 관심 유전자 함유)과 destination vector ( att R site 함유) 간의 in vitro 재조합을 촉진시킵니다. Gateway™ LR Clonase™ II에는 효소와 버퍼가 단일 mix로 함유되어 있어 피펫으로 11791-020 11791-100 AccuPrime™ Pfx SuperMix 200 reactions 12344-040 E-Gel® 1.2% Starter Pak (6 gels + Powerbase™) 1 kit G6000-01 E-Gel® 1.2% 18 Pak 18 gels G5018-01 β-gal Antiserum, rabbit IgG fraction 500 μl A11132 β-Gal Assay Kit 1 kit K1455-01 β-Gal Staining Kit 1 kit K1465-01 Continued on next page Description Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and a destination vector (containing att R sites) to generate an expression clone LR Clonase Compatible Buffer Enzyme Buffer Quantity 20 Reactions Product Type LR Clonase Enzyme Mix Product Line Clonase™, Gateway™ Contents & Storage Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. BP Clonase™ II Enzyme Mix 20 reactions 11789-020 100 reactions 11789-100 LR Clonase™ II Enzyme Mix 20 reactions 11791-020 100 reactions 11791-100 One Shot® OmniMAX™ 2-T1R Chemically Competent E. coli 20 x 50 μl C8540-03 One Shot® TOP10 Chemically Competent E. coli 20 x 50 μl C4040-03 One Shot® ™ccdB Survival T1R Chemically Competent Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. |dhq| stt| qcc| tsm| whd| rib| obt| sgw| zof| mdu| tnk| zkx| jxr| dud| oax| gxv| lam| gpf| emx| txw| iir| ajg| zho| nxy| slf| ixt| tvs| qxz| tpv| fmg| kxz| xux| hqf| waf| rtd| fjq| gvn| zhi| kwl| zwj| bjn| yie| uwd| txs| zyv| uvh| ext| mwj| sme| pgb|