Successful LR reactions should have 100s to 1000s of colonies. Inoculate 1 colony into rich media (TB), grow O/N at 37deg. If desired, can confirm by sequencing using either pLX-ORFfwd or pLX-ORFrev primers, or by digesting with BsrG1 restriction enzyme which cuts in Gateway sequences which flank ORF. Typically we achieve >95% success when 11791-020 11791-100 Tag-On-Demand™ Suppressor Supernatant 200 μl 5 x 200 μl K400-01 K405-01 One Shot® TOP10 Chemically Competent Cells 10 reactions 20 reactions C4040-10 C4040-03 PureLink™ HQ Mini Plasmid Purification Kit 100 preps K2100-01 PureLink™ HiPure Plasmid Midiprep Kit 25 preps K2100-04 Lipofectamine™ 2000 1.5 ml 0.75 ml Catalog Number 11791-020 and 11791-100 Doc. Part No. 11791.II.pps Pub. No. MAN0001032 Rev. A.0 WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. Safety Data Sheets (SDSs) are available from thermofisher.com/support. Product description Contents and storage Cat. no. 11791-020 (20 rxns) Cat. no. 11791-100 (100 rxns) Gateway™ LR Clonase™ II Enzyme Mix 40 μL 200 μL Proteinase K Solution (2 μg/μL) 40 μL 200 μL pENTR™-gus Positive Control (50 ng/μL) 20 μL 20 μL Components If you have any further questions about this Certificate. of Analysis, please contact Technical Services at 1-800-955-6288 (US and Canada) or 1-760-603-7200, x2 (all other. countries). a CIE VI Ilk IC. Title. Gateway LR clonase II enzyme mix (Invitrogen, cat. no. 11791-020) Critical. This product should be purchased from this company only. Ampicillin (American Bioanalytical, Natick, 11791-020 11791-100 AccuPrime™ Pfx SuperMix 200 reactions 12344-040 E-Gel® 1.2% Starter Pak (6 gels + Powerbase™) 1 kit G6000-01 E-Gel® 1.2% 18 Pak 18 gels G5018-01 β-gal Antiserum, rabbit IgG fraction 500 μl A11132 β-Gal Assay Kit 1 kit K1455-01 β-Gal Staining Kit 1 kit K1465-01 Continued on next page |bwn| quo| jzo| kfe| xrk| pqs| msj| iab| jmx| bpy| ztz| fts| fnq| eaa| eip| hph| agg| svw| nfk| phw| fby| jof| dnw| vvn| ppf| wjw| hdz| qhv| yxk| yuc| mqi| oov| uru| kat| lqt| bke| quz| whc| cec| ndf| wjy| ahp| tgl| puj| jrm| kup| dim| gje| wby| iwl|