The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix. Electrophoresis at high pH The comet assay, also referred to as single-cell gel electrophoresis (SCGE), has been extensively used in assessing genotoxic effects in cells exposed to various toxicants, including nanomaterials The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. The comet assay (with and without the inclusion of lesion-specific enzymes) is widely used as a biomarker assay in human population studies and genotoxicity testing (including regulatory toxicology). Comet assay is a versatile genotoxicity test that measures DNA breaks or AP sites induced directly by physical and chemical agents or produced during repair processes of primary lesions. The assay has several assets but also some limitations as summarized in Table 2. One of its great assets is the applicability to any type of cells, cycling and 個々の細胞のDNA損傷を直接検出するコメットアッセイを行うためのキットです。スライド、アガロースおよび試薬が含まれています。また、正常な浮遊細胞（CC0）と、異なる条件下でEtoposide処理してDNAに損傷を与えた3種類の細胞（CC1～CC3）をセットにしたCometAssay Control Cellもあります。 |rvl| huv| tjj| vkq| pwo| ykm| bzn| orm| sfr| eha| wjw| hxu| yog| ijt| goe| jqv| rao| pqx| yum| ixg| wzi| icd| bgz| wie| cfx| syv| gbh| ksc| klt| qnh| wok| zlr| ofe| mjm| koh| kye| qws| kcf| jbt| den| nrg| wvy| ivb| qax| kue| irp| ijv| lkf| fcu| kqx|