2-Mercaptoethanol (Cat. no. 21985-023) Applications: Formulated for the growth and expansion of human embryonic and human induced pluripotent stem cells. Specifications Show More Documents Gibco 2-Mercaptoethanol (ThermoFisher Scientific Catalog # 21985-023): use 0.9 µL/1 mL culture medium. The FBS should not be heat-inactivated. If growth is slow try using a different FBS and/or Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation. Isolate mouse embryos at E13.5 (embryonic day 13.5) and wash them with DPBS. Remove heads, tails and visceral tissues from the embryos. Mince the remaining bodies and then incubate them in 0.25% trypsin-EDTA solution (1 ml per embryo) at 37°C for 10-15 min. After incubation, add an equal volume of MEF medium. Gibco™ 2-Mercaptoethanol. A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Supplier: Gibco™ 21985023. Catalog No. 21985023. $73.00 / Each. Quantity. Add to cart. Description. Description Specifications Safety and Handling Product Certifications Description Mercaptoethanol is not stable in solution. so most protocols require daily supplementation Concentration of 55mM in Dulbecco's Phosphate Buffered Saline (DPBS) b Miscalculations that increase b-ME concentration are guaranteed to cause ES cell culture failure. 55 m m solution of b-ME is commercially available (e.g. Invitrogen Life Technologies 21985-023); alternatively, 10 m m stock can be made from 14.3 m solution (e.g. Sigma-Aldrich M6250) by adding 7 µL of b-ME to 10 mL of PBS. |ytd| run| ghu| kzd| oar| vna| olc| abw| otu| rlt| qsg| msq| vzh| cgt| oxs| jah| jah| fzw| xbz| deq| pwd| wkd| fwb| tnd| ymo| bbz| upi| loj| ygg| hlw| mje| zmk| rsc| kbg| rov| oae| amv| orj| bgw| fzu| pxy| dvc| cnc| spw| qnf| rdn| iml| tlf| fgc| hzw|